Recombinant Human SELP/selectin P/P-selectin Protein (His Tag)

Specifications

Catalog number

PKSH030758-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

Synonyms

CD62; CD62P; GMP140; GRMP; LECAM3; P-Selectin; PADGEM; PSEL

UniProt

P16109

Accession Number

AAN06828.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 771

Field of Research

Cardiovascular, Immunology, Stem Cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

81.3 kDa

Observed Molecular Weight

120 kDa

Species

Human