Recombinant Human SERPINB9/PI-9 Protein (Baculovirus, His Tag)

Specifications

Catalog number

PKSH030777

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SerpinB9; also known as Cytoplasmic antiproteinase 3; CAP-3; Peptidase inhibitor 9; SERPINB9 and PI-9; is a cytoplasm protein which belongs to theserpin family and Ov-serpin subfamily. Serpin-B9 (CAP-3 / PI-9) is the only known human intracellular inhibitor of granzyme B (GrB) ; the effector molecule in immunity against cytomegalovirus (CMV) and in renal allograft rejection. Serpin-B9 and SPI-6 expression in immune-privileged cells; APCs; and CTLs protects these cells against the actions of granzyme B; and when expressed in tumor cells or virally infected hepatocytes; confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 (CAP-3 / PI-9) in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin / granzyme pathway and then through the Fas / FasL pathway. Serpin-B9 (CAP-3 / PI-9) is selectively up-regulated in hepatocytes in response to infiltration of the liver by NK cells that express perforin and enzymatically active granzyme B. Upregulated expression of Serpin-B9 (CAP-3 / PI-9) in NSCLC cells may serve to protect them from apoptosis induced by GrB.

Synonyms

CAP3; Cytoplasmic antiproteinase 3; PI-9; Peptidase inhibitor 9; Serpin B9

UniProt

P50453

Accession Number

P50453

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Glu 2-Pro 376

Field of Research

Cell biology; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.5 kDa

Observed Molecular Weight

43 kDa

Species

Human