Recombinant Human UBE2A Protein (His Tag)

Specifications

Catalog number

PKSH030787

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A) ; encoded by human DNA repair genes HHR6A; belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene; a member of the family of ubiquitin-conjugating enzymes. In vivo; HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition; with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity; while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.

Synonyms

HR6A; RAD6 Homolog A; RAD6A; UBE2A; Ubiquitin Carrier Protein A; Ubiquitin-Conjugating Enzyme E2 A; Ubiquitin-Protein Ligase A; hHR6A

UniProt

P49459

Accession Number

P49459

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Cys 152

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.2 kDa

Observed Molecular Weight

18.5 kDa

Species

Human