Recombinant Human NEIL1 Protein (His Tag)

Specifications

Catalog number

PKSH030794

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NEIL1 is a member of DNA glycosylases. DNA glycosylases are a family homologous to the bacterial Fpg/Nei family. They play a role in base excision repair which is the mechanism by which damaged bases in DNA are removed and replaced. The first step of this process is catalyzed by DNA glycosylases. They remove the damaged nitrogenous base while leaving the sugar-phosphate backbone intact, creating an apurinic/apyrimidinic site, commonly referred to as an AP site. NEIL1 functions in base excision repair of DNA damaged by oxidation or by mutagenic agents. It acts as DNA glycosylase that recognizes and removes damaged bases. NEIL1 prefers to oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand and cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Abbreviation

NEIL1

Synonyms

NEI; FPG; NEIL; hFPG; NEIL1; FPG1; NEI1; hFPG1

UniProt

Q96FI4

Accession Number

AAH10876.1

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Ser 390

Field of Research

Epigenetics and Nuclear Signaling

Endotoxin

Please contact us for more information.

Purity

> 84 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45 kDa

Observed Molecular Weight

45 kDa

Species

Human