Recombinant Human Ube2L6 Protein (His Tag)

Specifications

Catalog number

PKSH030799

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

UBCH8; also known as UBE2L6; belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so; E2s differentiate effects on downstream substrates; either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level; it is present in natural killer cells.

Synonyms

RIG-B; Retinoic Acid-Induced Gene B Protein; UBCH8; UBE2L6; UbcH8; Ubiquitin Carrier Protein L6; Ubiquitin-Protein Ligase L6; Ubiquitin/ISG15-Conjugating Enzyme E2 L6

UniProt

O14933

Accession Number

O14933-1

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ser 153

Field of Research

Epigenetics and Nuclear Signaling, Cell Biology

Endotoxin

Please contact us for more information.

Purity

> 96 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.6 kDa

Species

Human