Recombinant Human APE1/APE Protein (His Tag)

Specifications

Catalog number

PKSH030851

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease; DNA 3' repair diesterase; 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously; the levels varied significantly; suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis; relatively high levels in the thymus; spleen; kidney and brain; and the lowest level in the liver in rats. However; the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain; especially in the hippocampal formation; to regulate some brain functions by redox-activation.

Synonyms

AP Endonuclease 1; APE; APE-1REF-1; APE1; APEN; APEX; APEX Nuclease; APEX1; APX; Apurinic-Apyrimidinic Endonuclease 1; DNA- (Apurinic or Apyrimidinic Site) Lyase; HAP1; REF1; Redox Factor-1

UniProt

P27695

Accession Number

P27695

Expression System

E.coli

Tag

N-His

Sequence

Pro2-Leu 318

Field of Research

Cardiovascular; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.0 kDa

Observed Molecular Weight

37 kDa

Species

Human