Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)

Specifications

Catalog number

PKSH030856

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic anhydrase 5B; also known as carbonate dehydratase VB; carbonic anhydrase VB; CA-VB and CA5B; is amember of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart; pancreas; kidney; placenta; lung; and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes; including respiration; calcification; acid-base balance; bone resorption; and the formation of aqueous humor; cerebrospinal fluid; saliva; and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA; which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine; L-adrenaline; L- and D-histidine; and L- and D-phenylalanine. It is inhibited by coumarins; sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt) . CA5B / CA-VB is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA) ; saccharin and Foscarnet (phosphonoformate trisodium salt) .

Synonyms

CA-VB; CA5B; Carbonate Dehydratase VB; Carbonic Anhydrase 5B Mitochondrial; Carbonic Anhydrase VB

UniProt

Q9Y2D0

Accession Number

Q9Y2D0

Expression System

E.coli

Tag

C-His

Sequence

Cys 34-Pro 317

Applications

Enzyme

Field of Research

Signal Transduction; Tags & Cell Markers; metabolism

Endotoxin

Please contact us for more information.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its esterase activity. The specific activity is > 150 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.0 kDa

Observed Molecular Weight

34 kDa

Species

Human