Recombinant Human CLIC4 Protein (His Tag)

Specifications

Catalog number

PKSH030859

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chloride intracellular channel protein 4; also known as Intracellular chloride ion channel protein p64H1 and CLIC4; is a member of the chloride channel CLIC family. It contains oneGST C-terminal domain. CLIC4 is a member of a family of intracellular chloride channels. It is regulated by p53; c-Myc; and tumor necrosis factor-alpha. CLIC4 is detected in epithelial cells from colon; esophagus and kidney (at protein level) . CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. CLIC4 could promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis) . Expression of CLIC4 is prominent in heart; kidney; placenta and skeletal muscle. Overexpression of CLIC4 in cancer cells inhibits tumor growth. Conversely; overexpression of CLIC4 in tumor stromal cells stimulates tumor growth. Thus; CLIC4 participates in normal and pathological processes and may serve as a useful target for therapies in disturbances of homeostasis and neoplastic transformation. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion; generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth.

Synonyms

CLIC4L; H1; MTCLIC; huH1; p64H1

UniProt

Q9Y696

Accession Number

Q9Y696-1

Expression System

E.coli

Tag

N-His

Sequence

Ala 2-Lys 253

Field of Research

Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction, Metabolism, Cancer

Endotoxin

Please contact us for more information.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.6 kDa

Observed Molecular Weight

30 kDa

Species

Human