Recombinant Human Fumarase/FH Protein (His Tag)

Specifications

Catalog number

PKSH030901

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event.

Synonyms

FH; FMRD; Fumarase; Fumarate Hydratase Mitochondrial; HLRCC; LRCC; MCL; MCUL1

UniProt

P07954

Accession Number

P07954-1

Expression System

E.coli

Tag

C-His

Sequence

Ala 45-Lys 510

Applications

Enzyme

Field of Research

Signal Transduction, Cancer, Metabolism

Endotoxin

Please contact us for more information.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to transform 1μmole of Fumarate to L-malate per minute at pH 7.5 at 37°C. Specific activity is > 25 unit/mg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.0 kDa

Observed Molecular Weight

45 kDa

Species

Human