Recombinant Human IDH1 Protein (E.coli, His Tag)

Specifications

Catalog number

PKSH030920-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.

Synonyms

Cytosolic NADP-Isocitrate Dehydrogenase; IDH; IDH1; IDP; Isocitrate Dehydrogenase [NADP] Cytoplasmic; NADP (+) -Specific ICDH; Oxalosuccinate Decarboxylase; PICD

UniProt

O75874

Accession Number

O75874

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Leu 414

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

48.0 kDa

Observed Molecular Weight

43 kDa

Species

Human