Recombinant Human HEXA Protein (Subunit A, His Tag)

Specifications

Catalog number

PKSH030928

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G (1) /S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.

Synonyms

TSD

UniProt

P06865

Accession Number

AAD13932.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Thr 529

Applications

Enzyme

Field of Research

Tags & Cell Markers, Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-Glc-NAc) . The specific activity is > 1, 250 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

59.7 kDa

Observed Molecular Weight

64 kDa

Species

Human