Recombinant Human PTGS2/COX2/PGHS-2 Protein (His Tag)

Specifications

Catalog number

PKSH030929

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PTGS2, also known as COX-2, is s component of Prostaglandin-endoperoxide synthase (PTGS) . PTGS, also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS2 is over expressed in many cancers. The overexpression of PTGS2 along with increased angiogenesis and GLUT-1 expression is significantly associated with gallbladder carcinomas. Furthermore the product of COX-2, PGH2 is converted by prostaglandin E2 synthase into PGE2, which in turn can stimulate cancer progression. Consequently inhibiting COX-2 may have benefit in the prevention and treatment of these types of cancer. PTGS2 is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. It mediates the formation of prostaglandins from arachidonate and may have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Synonyms

COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2

UniProt

P35354

Accession Number

NP_000954.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Leu 604

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

68.5 kDa

Observed Molecular Weight

66 kDa

Species

Human