Recombinant Human c-KIT/CD117 Protein (aa 540-972, His & GST Tag)

Specifications

Catalog number

PKSH030938

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor; also known as stem cell factor) . c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily; tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs; c-Kit staining is typically cytoplasmic; with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors; mast cell disease; acute myelogenous lukemia; and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML) . AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

Synonyms

CD117; Mast/stem cell growth factor receptor Kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; SCFR; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

UniProt

P10721

Accession Number

P10721-2

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Thr 540-Val 972

Field of Research

Immunology, Tags & Cell Markers, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

76.8 kDa

Observed Molecular Weight

68 kDa

Species

Human