Recombinant Human SUV420H2 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030951

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Histone-lysine N-methyltransferase SUV420H2, also known as Suppressor of variegation 4-20 homolog 2, Su (var) 4-20 homolog 2, Lysine N-methyltransferase 5C, SUV420H2 and KMT5C, is nucleus protein which belongs to thehistone-lysine methyltransferase family and Suvar4-20 subfamily. SUV420H2 is a histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. SUV420H2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) . FRAP experiments reveal that SUV420H2 is strongly associated to pericentric heterochromatin. The fraction of SUV420H2 captured and characterized by TAP/MS is a soluble fraction which may be in a stable association with HP1. SUV420H2 may be recruited to heterochromatin in association with HP1, and stably maintained at its heterochromatin sites in an HP1-independent fashion.

Synonyms

KMT5C

UniProt

Q86Y97

Accession Number

NP_116090.2

Expression System

E.coli

Tag

N-His-GST

Sequence

Gly 2-Leu 280

Field of Research

Epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

60.0 kDa

Observed Molecular Weight

60 kDa

Species

Human