Recombinant Human RPE Protein (His Tag)

Specifications

Catalog number

PKSH030953

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha) (8) -barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE) ; orotidine 5'-monophosphate decarboxylase (OMPDC) ; and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC) . Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation; or origin; site; at which DNA synthesis begins. In enzymology; a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence; RPE has one substrate; L-ribulose 5-phosphate; and one product; L-xylulose 5-phosphate. RPE belongs to the family of isomerases; specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase; UlaE; and SgaU.

Synonyms

HUSSY-17; RPE; RPE2-1; Ribulose-5-Phosphate-3-Epimerase; Ribulose-Phosphate 3-Epimerase

UniProt

Q96AT9

Accession Number

NP_954699.1

Expression System

HEK293 Cells

Tag

N-His

Sequence

Ala 2-Arg 228

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.0 kDa

Observed Molecular Weight

30 kDa

Species

Human