Recombinant Human EphB1/EPHT2 Protein (aa 1-540, His Tag)

Specifications

Catalog number

PKSH030955

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs) . Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Synonyms

EHK-3; EHK3; EK11; EPH Homology Kinase 3; EPH-Like Kinase 11; EPHA7; Ephrin Type-A Receptor 7; HEK11; hEK11

UniProt

P54762

Accession Number

P54762-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 540

Applications

ELISA

Field of Research

Cardiovascular, Neuroscience, Signal Transduction, Stem Cells, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB1-Fc2h with a linear ranger of 3. 125-200 ng/mL. Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

60.0 kDa

Observed Molecular Weight

60 kDa

Species

Human