Recombinant Human ROBO4 Protein (His Tag)

Specifications

Catalog number

PKSH030956

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Roundabout homolog 4; also known as magic roundabout and ROBO4 is a member of theimmunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor types. ROBO4 contains twofibronectin type-III domains and twoIg-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins; at least for SLIT2; and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.

Synonyms

Magic roundabout; ROBO4; Roundabout homolog 4; UNQ421/PRO3674

UniProt

Q8WZ75

Accession Number

Q8WZ75-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Glu 469

Field of Research

Cardiovascular, Neuroscience, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.0 kDa

Observed Molecular Weight

55-60 kDa

Species

Human