Recombinant Human CASK Kinase Protein (His & GST Tag)

Specifications

Catalog number

PKSH030973

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Peripheral plasma membrane protein CASK; also known as calcium/calmodulin-dependent serine protein kinase; CASK and LIN2; is a nucleus; cytoplasm and cell membrane protein which belongs to theMAGUK family. CASK / LIN2 contains oneguanylate kinase-like domain; twoL27 domains; onePDZ (DHR) domain; oneprotein kinase domain and oneSH3 domain. CASK / LIN2 is ubiquitously expressed. Expression of CASK / LIN2 is significantly greater in brain relative to kidney; lung; and liver and in fetal brain and kidney relative to lung and liver. CASK / LIN2 is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. CASK / LIN2 contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. It binds to cell-surface proteins; including amyloid precursor protein; neurexins and syndecans. CASK / LIN2 may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Defects in CASK are also the cause of FG syndrome type 4 which is an X-linked disorder characterized by mental retardation; relative macrocephaly; hypotonia and constipation.

Synonyms

CAGH39; CAMGUK; CMG; FGS4; LIN2; MICPCH; MRXSNA; TNRC8

UniProt

O14936

Accession Number

O14936-4

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Ala 2-Tyr 898

Field of Research

Neuroscience, Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 82 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

130 kDa

Observed Molecular Weight

120 kDa

Species

Human