Recombinant Human XPNPEP2 Protein (His Tag)

Specifications

Catalog number

PKSH030976

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds; which are common to several collagen degradation products; neuropeptides; vasoactive peptides; and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2; the X-linked gene that encodes membranous aminopeptidase P (APP) ; has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family; of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation; suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF) .

Synonyms

Aminoacylproline Aminopeptidase; Membrane-Bound APP; Membrane-Bound AmP; Membrane-Bound Aminopeptidase P; X-Pro Aminopeptidase 2; XPNPEP2; Xaa-Pro Aminopeptidase 2; mAmP

UniProt

O43895

Accession Number

O43895

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 650

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys (2-Aminobenzoyl) Pro-Pro-pNitroanilide (K (Abz) PP-pNA) . The specific activity is > 300 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

72.0 kDa

Species

Human