Recombinant Human Trypsin-3/PRSS3 Protein (His Tag)

Specifications

Catalog number

PKSH030980

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Trypsin-3, also known as Trypsin III, brain trypsinogen, Serine protease 3 and PRSS3, is a secreted protein which belongs to thepeptidase S1 family. Trypsin-3 / PRSS3 is expressed is in pancreas and brain. It contains onepeptidase S1 domain. Trypsin-3 / PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP) . A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3 / PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression, which is correlated with promoter hypermethylation, in esophageal squamous cell carcinoma and gastric adenocarcinoma.

Synonyms

MTG; PRSS4; RP11-176F3.3; T9; TRY3; TRY4

UniProt

P35030

Accession Number

P35030-3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 247

Applications

Enzyme

Field of Research

Cell Biology, Cancer, Signal Transduction, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (AnaSpec, Catalog#27114) . The specific activity is > 4, 000 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.6 kDa

Observed Molecular Weight

33 kDa

Species

Human