Recombinant Human Kallikrein 4/KLK4 Protein (His Tag)

Specifications

Catalog number

PKSH030981

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein-like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17 and EMSP1, is a secreted protein which belongs to thepeptidase S1 family and Kallikrein subfamily. Kallikrein-4 / KLK4 is a serine protease expressed during enamel maturation, and proteolytic processing of the enamel matrix by KLK4 is critical for proper enamel formation. Kallikrein-4 / KLK4 contains onepeptidase S1 domain. Kallikrein-4 / KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-20) . The late protease is kallikrein 4 (KLK4) . The principle functions of MMP-20 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.

Synonyms

AI2A1; ARM1; EMSP; EMSP1; Enamel Matrix Serine Proteinase 1; KLK-L1; KLK4; Kallikrein-4; Kallikrein-Like Protein 1; PRSS17; PSTS; Prostase; Serine Protease 17; kallikrein

Accession Number

NP_004908.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 254

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. (R&D Systems, Catalog # ES011) . The specific activity is > 250 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.8 kDa

Observed Molecular Weight

30-35 kDa

Species

Human