Recombinant Human Caspase-14/CASP14 Protein (His Tag)

Specifications

Catalog number

PKSH030982

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases (caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.

Synonyms

CASP-14; CASP14; Caspase-14; MGC119078; MGC119079

UniProt

B2CIS9

Accession Number

NP_036246.1

Expression System

E.coli

Tag

N-His

Sequence

Ser 2-Gln 242

Applications

ELISA

Field of Research

Cell biology

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind biotinylated Cynomolgus IL18 in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.5 kDa

Observed Molecular Weight

30 kDa

Species

Human