Recombinant Human Semaphorin 4D/SEMA4D Protein (His Tag)

Specifications

Catalog number

PKSH030994

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Synonyms

A8; BB18; C9orf164; CD100; GR3; M-sema-G; SEMAJ; Semaphorin-4D; coll-4

UniProt

Q92854

Accession Number

Q92854-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Arg 734

Applications

ELISA

Field of Research

Immunology, Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human SEMA4D-His at 10 μg/ml (100 μl/well) can bind mouse PTPRC-Fc with a linear range of 0.625-5 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

80.7 kDa

Observed Molecular Weight

110 kDa

Species

Human