Recombinant Human PDE9A Protein (His & GST Tag)

Specifications

Catalog number

PKSH031015

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

High affinity cGMP-specific 3';5'-cyclic phosphodiesterase 9A; also known as PDE9A; is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis; brain; small intestine; skeletal muscle; heart; lung; thymus; spleen; placenta; kidney; liver; pancreas; ovary and prostate) except blood. Highest levels of PDE9A is in brain; heart; kidney; spleen; prostate and colon. IsoformPDE9A12is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain; with the highest expression observed in cerebellar Purkinje cells.PDE9A is the only cGMP-specific PDE with significant expression in the forebrain; and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP; which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.

Synonyms

HSPDE9A2

UniProt

O76083

Accession Number

O76083-2

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Ala 533

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

89.5 kDa

Observed Molecular Weight

75 kDa

Species

Human