Recombinant Human PCBP1 Protein (His Tag)

Specifications

Catalog number

PKSH031057

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Poly (rC) -binding protein 1, also known as Heterogeneous nuclear ribonucleoprotein E1, Alpha-CP1, Nucleic acid-binding protein SUB2.3 and PCBP1, is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. PCBP1 is loosely bound in the nucleus. It may shuttle between the nucleus and the cytoplasm. It is abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. PCBP1 is widely expressed in many human tissues and involved in regulation of transcription, transportation process, and function of RNA molecules. PCBP1 plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. PCBP1 acts as a negative regulator of CD44 variants splicing in HepG2 cells, and loss of PCBP1 in human hepatic tumor contributes to the formation of a metastatic phenotype.

Synonyms

HEL-S-85; HNRPE1; HNRPX; hnRNP-E1; hnRNP-X

UniProt

Q15365

Accession Number

NP_006187.2

Expression System

E.coli

Tag

N-His

Sequence

Asp 2-Cys 356

Field of Research

Epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 87 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

38.3 kDa

Observed Molecular Weight

42 kDa

Species

Human