Recombinant Human Neuroligin 1/NLGN1 Protein (His Tag)

Specifications

Catalog number

PKSH031062

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neuroligin 1 (NLGN1) belongs to the type-B carboxylesterase/lipase family; is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors; channels; and signal-transduction molecules to synaptic sites of cell adhesion. Neuroligins consist of five members (NLGN1; NLGN2; NLGN3; NLGN4 and NLGN4Y) ; which interact with beta-neurexins and this interaction is involved in the formation of functional synapses. The extracellular domain of functional Neuroligin 1 associates as a dimer when analyzed by sedimentation equilibrium. Neuroligin 1 has a unique N-linked glycosylation pattern in the neuroligin family; and glycosylation and its processing modify neuroligin activity. Neuroligin 1 is a potent trigger for the de novo formation of synaptic connections; and it has recently been suggested that it is required for the maturation of functionally competent excitatory synapses. The persistent expression of Neuroligin 1 is required for the maintenance of NMDAR-mediated synaptic transmission; which enables normal development of synaptic plasticity and long-term memory in the amygdala of adult animals.

Synonyms

KIAA1070; NL1; NLGN1; Neuroligin-1

UniProt

Q8N2Q7

Accession Number

NP_055747.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 677

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

72.0 kDa

Observed Molecular Weight

85-95 kDa

Species

Human