Recombinant Human CD111/Nectin-1 Protein (His Tag)

Specifications

Catalog number

PKSH031065

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV) . CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

Synonyms

CD111; ED4; HIgR; HV1S; HVEC; Herpes Virus Entry Mediator C; Herpesvirus Entry Mediator C; Herpesvirus Ig-Like Receptor; HveC; Nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; Poliovirus Receptor-Related Protein 1; SK-12; nectin-1

UniProt

Q15223

Accession Number

NP_002846.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr 334

Applications

ELISA

Field of Research

Microbiology; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.4-800 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.4 kDa

Observed Molecular Weight

45-50 kDa

Species

Human