Recombinant Human Neuregulin-1/NRG1-β1 Protein (EGF Domain, Fc Tag)

Specifications

Catalog number

PKSH031068-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development; neurotransmission; and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems; and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder; with etiology likely due to epistasis.

Synonyms

EGF; GGF; HGL; HRG1-beta 1; HRGA; NDF; NRG1; NRG1-beta 1; Neuregulin-1 beta 1; Pro-neuregulin-1; SMDF

UniProt

Q02297

Accession Number

Q02297-6

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Thr 176-Lys 246

Applications

ELISA

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 86 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized Rhesus ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.58 μg/mL. 2. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.7 kDa

Observed Molecular Weight

38 kDa

Species

Human