Recombinant Human AKR1A1 Protein (His Tag)

Specifications

Catalog number

PKSH031070

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD (P) -binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

Synonyms

ALDR1; ALR; ARM; DD3; HEL-S-6

UniProt

P14550

Accession Number

P14550

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Tyr 325

Field of Research

Signal Transduction; Cell biology; Cancer; metabolism

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.0 kDa

Observed Molecular Weight

39 kDa

Species

Human