Recombinant Human SULT1B1 Protein (His Tag)

Specifications

Catalog number

PKSH031074

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sulfotransferase family cytosolic 1B member 1; also known as Sulfotransferase 1B1; Sulfotransferase 1B2; Thyroid hormone sulfotransferase; SULT1B1 and ST1B2; is a cytoplasm protein which belongs to thesulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones; neurotransmitters; drugs; and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1B1 is highly expressed in the liver; peripheral blood leukocytes; colon (mucosal lining) ; small intestine (jejunum) and spleen. A lesser expression of SULT1B1 was observed in the lung; placenta and thymus. SULT1B1 catalyzes the sulfate conjugation of many hormones; neurotransmitters; drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds; and therefore their renal excretion; but it can also result in bioactivation to form active metabolites. SULT1B1 sulfates dopamine; small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones; including 3;3'-diiodothyronine; triidothyronine; reverse triiodothyronine and thyroxine.

Synonyms

ST1B1; ST1B2; SULT1B1; SULT1B2; Sulfotransferase 1B1; Sulfotransferase 1B2; Sulfotransferase Family Cytosolic 1B Member 1; Thyroid Hormone Sulfotransferase

UniProt

O43704

Accession Number

NP_055280.2

Expression System

E.coli

Tag

N-His

Sequence

Leu 2-Ile 296

Applications

Enzyme

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is > 40 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.7 kDa

Observed Molecular Weight

34 kDa

Species

Human