Recombinant Human ICOS Ligand/ICOSL Protein (His Tag)

Specifications

Catalog number

PKSH031085

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.

Synonyms

B7 Homolog 2; B7-H2; B7-Like Protein Gl50; B7-Related Protein 1; B7H2; B7RP-1; B7RP1; CD275; ICOS Ligand; ICOSL; ICOSLG; KIAA0653

UniProt

A0N0L8

Accession Number

NP_056074.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 258

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1. 6-200 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.0 kDa

Observed Molecular Weight

50-60 kDa

Species

Human