Recombinant Human METTL1 Protein (His Tag)

Specifications

Catalog number

PKSH031101

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TRNA (guanine-N (7) -) -methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46) -methyltransferase and METTL1, is a nucleus protein which belongs to themethyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species) . Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

Synonyms

C12orf1; TRM8; TRMT8; YDL201w

UniProt

Q9UBP6

Accession Number

NP_005362.3

Expression System

E.coli

Tag

N-His

Sequence

Asp 32-Gln 265

Field of Research

Epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.0 kDa

Observed Molecular Weight

30 kDa

Species

Human