Recombinant Human Coagulation Factor IX/F9 Protein (His Tag)

Specifications

Catalog number

PKSH031109

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B (HEMB) which also known as Christmas disease.

Synonyms

Christmas factor; Coagulation factor 9; Coagulation factor IX; Coagulation factor IXa heavy chain; Coagulation factor IXa light chain; F9; FIX; HEMB; P19; PTC; Plasma thromboplastin component; THPH8

UniProt

P00740

Accession Number

AAB59620.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr 461

Applications

Enzyme

Field of Research

Cardiovascular, Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the peptide substrate, Z-D-Arg-Gly-Arg-pNA. The specific activity is > 20pmols/min/ug.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.0 kDa

Observed Molecular Weight

60-80 kDa

Species

Human