Recombinant Human IL-21R Protein (His Tag)

Specifications

Catalog number

PKSH031111

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-21 receptor; also known as IL-21 receptor; IL-21R; Novel interleukin receptor; IL21R and NILR; is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 4 subfamily. Interleukin-21 (IL-21) belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL-21R) and the common cytokine receptor gamma chain (gamma (C) ) . The IL-21R is discovered as a novel member of the class-I-cytokine-receptor family and is selectively expressed in lymphoid tissues. IL-21R shows strong sequence homologies to the interleukin-4 receptor alpha chain gene (IL-4RA) . The WSXWS motif of IL-21R appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif of IL-21R is required for JAK interaction and / or activation. The IL-21R is widely distributed on lympho-haematopoietic cells and IL21 impacts a number of cell types; including CD8+ memory T cells; NK cells and subsets of CD4 memory T cells. Increased IL21 production is characteristic of certain autoimmune diseases and is likely to contribute to autoantibody production as well as pathological features of autoimmune disease. The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterised by overproduction of pathogenic autoantibodies.

Abbreviation

IL-21R

Synonyms

UNQ; IL21R; CD360; NILR; CD360.; IL-21 receptor; IL-21R; Interleukin-21 receptor; UNQ3121; PRO10273; NILR

UniProt

Q9HBE5

Accession Number

Q9HBE5-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 236

Applications

Cell culture; ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind human IL-21 in a functional ELISA. 2. Measured by its ability to inhibit IL21-induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 1-5 ng/ml in the presence of 10 ng/ml of recombinant human IL2 3. Measured by its ability to inhibit IL21-induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 50-200 pg/mL in the presence of 1 ng/ml of recombinant human IL21.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.5 kDa

Species

Human