Recombinant Human CMA1/Chymase 1 Protein (His Tag)

Specifications

Catalog number

PKSH031112

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chymotrypsin C (abbreviated for CTRC), also known as caldecrin or elastase4, is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis, chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion, however, the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC, whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed.

Synonyms

CYH; MCT1; chymase

UniProt

P23946

Accession Number

P23946

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Asn 247

Field of Research

Signal Transduction; Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

26.6 kDa

Observed Molecular Weight

33 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

Species

Human