Recombinant Human B3GNT2 Protein (Fc Tag)

Specifications

Catalog number

PKSH031125

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein which prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increases poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.

Synonyms

B3GN-T2; B3GNT; B3GNT-2; B3GNT1; BETA3GNT; BGNT2; BGnT-2

UniProt

Q9NY97

Accession Number

Q9NY97-1

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Lys29-Cys397

Field of Research

Tags & Cell Markers

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

71.2 kDa

Observed Molecular Weight

112-120 kDa

Species

Human