Recombinant Human SULT1A3 Protein (His Tag)

Specifications

Catalog number

PKSH031128

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SULT1A3 belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are different in their tissue distributions and substrate specificities while their gene structure (number and length of exons) is similar. SULT1A3 gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16; this gene and SULT1A4 arose from a segmental duplication. It is the most centromeric of the four sulfotransferase genes. Exons of this gene overlap with exons of a gene that encodes a protein containing GIY-YIG domains (GIYD1) . SULT1A3 is expressed in liver, colon, kidney, lung, brain, spleen, small intestine, placenta and leukocyte. SULT1A3 is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Synonyms

Aryl Sulfotransferase 1A3/1A4; Catecholamine-Sulfating Phenol Sulfotransferase; HAST3; M-PST; Monoamine-Sulfating Phenol Sulfotransferase; Placental Estrogen Sulfotransferase; ST1A3/ST1A4; Sulfotransferase 1A3/1A4; Sulfotransferase Monoamine-Preferring; Thermolabi

UniProt

P0DMM9

Accession Number

NP_808220.1

Expression System

E.coli

Tag

N-His

Sequence

Glu2-Leu295

Applications

Enzyme

Endotoxin

Please contact us for more information.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is > 150 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.5 kDa

Observed Molecular Weight

34 kDa

Species

Human