Recombinant Human RHEB Protein (His Tag)

Specifications

Catalog number

PKSH031137

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored; cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form; and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped; two on chromosome 10 and one on chromosome 22.

Synonyms

GTP-Binding Protein Rheb; RHEB; RHEB2; Ras Homolog Enriched in Brain

UniProt

Q15382

Accession Number

Q15382

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Met 1-Cys181

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.4 kDa

Observed Molecular Weight

21 kDa

Species

Human