Recombinant Human EphA4 Protein (His & Fc Tag)

Specifications

Catalog number

PKSH031153

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor− mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs) . The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Synonyms

EK8; EPH-like kinase 8; EPHA4; Ephrin type-A receptor 4; HEK8; SEK; TYRO1; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK; hEK8

UniProt

P54764

Accession Number

NP_004429.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Thr 547

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human EPHA5 at 20 μg/ml (100 μl/well) can bind human EFNA4-Fc with a linear ranger of 1. 28-32 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

86.5 kDa

Observed Molecular Weight

100-110 kDa

Species

Human