Recombinant Human Autotaxin/ENPP2 Protein (aa 49-863, His Tag)

Specifications

Catalog number

PKSH031157-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

Synonyms

ATX; ATX-X; ATXFLJ26803; Autotaxin; E-NPP 2; EC 3.1.4.39; ENPP2; LysoPLD; NPP2; PD-IALPHA; PDNP2; PDNP2NPP2; autotaxin-t; ectonucleotide pyrophosphatase/phosphodiesterase 2

UniProt

Q13822

Accession Number

AAH34961.1

Expression System

HEK293 Cells

Tag

N-His

Sequence

Asp 49-Ile 863

Field of Research

Cardiovascular, Neuroscience, Signal Transduction, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

96.0 kDa

Observed Molecular Weight

120-130 kDa

Species

Human