Recombinant Human GPNMB Protein (aa 1-474, His Tag)

Specifications

Catalog number

PKSH031159

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

GPNMB belongs to the PMEL / NMB family; also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 (HGFIN) ; is a transmembrane glycoprotein that is expressed in numerous cells; including osteoclasts; macrophages; dendritic cells; and tumor cells. It is suggested to influence osteoblast maturation; cell adhesion and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization; and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin as a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.

Synonyms

GPNMB; HGFIN; NMB; Osteoactivin; Transmembrane Glycoprotein HGFIN; Transmembrane Glycoprotein NMB

UniProt

Q14956

Accession Number

Q14956-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 474

Field of Research

Immunology, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.1 kDa

Species

Human