Recombinant Human PRDM2/RIZ1 Protein

Specifications

Catalog number

PKSH031203

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PR domain containing 2; with ZNF domain (PRDM2) ; also known as zinc finger protein RIZ; is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain; which shares sequence homology with an independently described sequence motif; the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain; and is highly expressed in retinoblastoma cell lines and in brain tumors; as well as in a number of other cell lines and in brain; heart; skeletal muscle; liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3; and is identified as a tumor suppressor. It is reported that intact PR (SET) sequence is required for tumor suppression functions; mutations in the PR domain caused activity reduction in human cancers. Also; S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition; PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3.

Synonyms

HUMHOXY1; KMT8; MTB-ZF; RIZ; RIZ1; RIZ2

UniProt

Q13029

Accession Number

NP_036363.2

Expression System

E.coli

Tag

None

Sequence

Met 1-Ala 200

Field of Research

Neuroscience; Cancer; epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 96 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23 kDa

Observed Molecular Weight

26 kDa

Species

Human