Recombinant Human 15-PGDH Protein (His Tag)

Specifications

Catalog number

PKSH031209

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor inmany species and regulate uterine contractility and cervicaldilatation. Therefore, the regulation of prostaglandin outputby PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activitypatterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR), cranioosteoarthropathy (COA), and isolated congenital nail clubbing.

Synonyms

15-Hydroxyprostaglandin Dehydrogenase [NAD (+) ];15-PGDH; HPGD; PGDH; PGDH1; PHOAR1; Prostaglandin Dehydrogenase 1; SDR36C1

UniProt

P15428

Accession Number

NP_000851.2

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Gln 266

Applications

Enzyme

Field of Research

Signal Transduction; immunology

Endotoxin

Please contact us for more information.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the production of NADH during the oxidation of PGF2α. The specific activity is > 1, 500 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.7 kDa

Observed Molecular Weight

27 kDa

Species

Human