Recombinant Human B7-H3/CD276 Protein (His Tag)

Specifications

Catalog number

PKSH031218-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Synonyms

B7 homolog 3; B7-H3; B7H34Ig-B7-H3; CD276; CD276 antigen; CD276 molecule; Costimulatory molecule

UniProt

Q5ZPR3

Accession Number

Q5ZPR3-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr 461

Field of Research

Immunology, Cancer, Stem Cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

48.0 kDa

Observed Molecular Weight

73 kDa

Species

Human