Recombinant Human SH2D1A/SAP Protein (His Tag)

Specifications

Catalog number

PKSH031236

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SH2 domain-containing protein 1A (SH2D1A / SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH2) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150 / SLAM, CD84, CD229 / Ly-9, and CD244 / 2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL) -like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome.

Synonyms

DSHP; Duncan Disease SH2-Protein; EBVS; IMD5; LYP; MTCP1; SAP/SH2D1A; SH2 Domain-Containing Protein 1A; SH2D1A; SLAM-Associated Protein; Signaling Lymphocytic Activation Molecule-Associated Protein; T-Cell Signal Transduction Molecule SAP; XLP; XLPD; XLPD1

UniProt

O60880

Accession Number

NP_002342.1

Expression System

E.coli

Tag

N-His

Sequence

Pro 2-Lys 97

Applications

ELISA

Field of Research

Cardiovascular; immunology

Endotoxin

Please contact us for more information.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind recombinant human SLAMF1, human SLAMF6, mouse SLAMF6, mouse CD84 in functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.6 kDa

Observed Molecular Weight

14 kDa

Species

Human