Recombinant Human S100A9 Protein (His Tag)

Specifications

Catalog number

PKSH031239

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calcium-binding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide. It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Synonyms

60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14; NIF; P14

UniProt

P06702

Accession Number

NP_002956.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Pro 114

Applications

Cell culture

Field of Research

Signal Transduction; Cell biology; Cancer; epigenetics and nuclear signal; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA. 2. Measured by its ability to inhibit proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-30 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.6 kDa

Observed Molecular Weight

16 kDa

Species

Human