Recombinant Human S100A8 Protein (E.coli, His Tag)

Specifications

Catalog number

PKSH031246

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

S100A8 is a member of the S100 protein family containing 2EF-hand calcium-binding motifs. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of S100A8 protein is associated with various diseases and cancers. The heterodimeric S100 protein complex S100A8/A9 which has been shown to be involved in inflammatory and neoplastic disorders. The complex can induce cell proliferation, or apoptosis, inflammation, collagen synthesis, and cell migration. S100A8/A9 has emerged as important pro-inflammatory mediator in acute and chronic inflammation. More recently, increased S100A8 and S100A9 levels were also detected in various human cancers, presenting abundant expression in neoplastic tumor cells as well as infiltrating immune cells. On the one hand, S100A8/A9 is a powerful apoptotic agent produced by immune cells, making it a very fascinating tool in the battle against cancer. It spears the risk to induce auto-immune response and may serve as a lead compound for cancer-selective therapeutics. In contrast, S100A8/A9 expression in cancer cells has also been associated with tumor development, cancer invasion or metastasis. Altogether, its expression and potential cytokine-like function in inflammation and in cancer suggests that S100A8/A9 may play a key role in inflammation-associated cancer.

Synonyms

60B8AG; CAGA; CFAG; CGLA; CP-10; Calgranulin-A; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; MRP-8; MRP8; NIF; P8; Protein S100-A8; S100A8

UniProt

P05109

Accession Number

NP_002955.2

Expression System

E.coli

Tag

N-His

Sequence

Leu 2-Glu 93

Applications

Cell culture

Field of Research

Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind recombinant human S100A9 in a functional ELISA. 2. This product displays no activity in cell-based assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.2 kDa

Observed Molecular Weight

12.2 kDa

Species

Human