Recombinant Human ABHD4 Protein (His Tag)

Specifications

Catalog number

PKSH031257-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Abhydrolase domain containing 4 (ABHD4), also known as alpha/beta-hydrolase 4 (ABH4), or lyso-N-acylphosphatidylethanolamine lipase, which belongs to the ABHD4/ABHD5 subfamily of peptidase S33 family. Abhydrolase domain containing (ABHD) gene was a small group belongs to alpha/beta hydrolase superfamily. Known members of this group are all found to be involved in important biochemical processes and related to various diseases. The alpha/beta-hydrolase 4 (ABH4) is a lysophospholipase/phospholipase B that selectively hydrolyzes N-acyl phosphatidylethanolamines (NAPEs) and lysoNAPEs. ABH4 accepts lysoNAPEs bearing both saturated and polyunsaturated N-acyl chains as substrates and displays a distribution that closely mirrors lysoNAPE-lipase activity in mouse tissues. The existence of an NAPE-PLD-independent route for NAE biosynthesis and suggest that ABH4 plays a role in this metabolic pathway by acting as a (lyso) NAPE-selective lipase.

Synonyms

ABH4

UniProt

Q8TB40

Accession Number

NP_071343.2

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Met 1-Asp 342

Field of Research

Signal Transduction; Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 82 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.0 kDa

Observed Molecular Weight

40 kDa

Species

Human