Recombinant Human R-Spondin 1/RSPO1 Protein (aa 1-146, His Tag)

Specifications

Catalog number

PKSH031268-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

Synonyms

CRISTIN3; FLJ40906; R-spondin; R-spondin1; RP11-566C13.1; RP23-325M14.2; RSPO Rspo1; RSPO1; Roof plate-specific spondin-1; Rspondin

UniProt

Q2MKA7

Accession Number

NP_001033722.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 146

Applications

ELISA

Field of Research

Developmental Biology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14 kDa

Observed Molecular Weight

23 kDa

Species

Human