Recombinant Human CEACAM5 Protein (His Tag)

Specifications

Catalog number

PKSH031272-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CEACAM5; also known as CEA or D66e; belongs to the large CEACAM subfamily of immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells; and is synthesized as a glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus; followed by six Ig-like C2-type domain and a GPI anchor; and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker; since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition; CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli; and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.

Synonyms

CD66e; CEA

Accession Number

NP_004354.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ala 685

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

72.8 kDa

Observed Molecular Weight

100-110 kDa

Species

Human